加入收藏
百奥莱博公司客服电话百奥莱博QQ百奥莱博QQ百奥莱博QQ
百奥莱博公司微信服务号

产品目录

重组人UBE2C(带His标签)图片
产品货号:
GS4872
中文名称:
重组人UBE2C(带His标签)
英文名称:
Recombinant Human Ubiquitin Conjugating Enzyme E2 C, His
产品规格:
10μg
发货周期:
1~3天
产品价格:
询价
分子量:Approximately 21.1kDa,a single non-glycosylated polypeptide chain containing 179 amino acids(a.a.)of human UBE2C/UBCH10 and 12 a.a.vector sequence including 6×His tag at N-terminus.
储存条件:冷冻(-20℃)

概述

Ubiquitin Conjugating Enzyme E2 C belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2C gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.The UBE2C catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex,and therefore,plays an important role in the control of the cell exit from mitosis.This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle.In addition,UBCH10 bears homology to yeast PAS2,a gene that is essential for biogenesis of peroxisomes.UBCH10 is useful for in vitro ubiquitinylation reactions.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观Sterile Filtered White lyophilized(freeze-dried)powder.
纯度>95%by SDS-PAGE and HPLC analyses.
来源Escherichia coli.
生物活性Data is not available.
成分Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素Less than 1 EU/μg of rHuUBE2C,His as determined by LAL method.
复溶We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P

相关搜索:重组人UBE2C(带His标签)UBE2C, His-tagged, Human
首页 |  关于我们 |  联系我们 |  在线咨询 |  网站地图
北京百奥莱博科技有限公司 京ICP备14007103号-3